Recombinant Human Anti-IAV HA Antibody (5J8)
CAT#: PABL-207
Recombinant Human Antibody (5J8) is capable of binding to IAV HA, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-IAV HA mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-IAV HA mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition.
Published Data
Tested Data
FuncS
Figure 1 Therapeutic efficacy of MAb 5J8 against disease caused bythe 1918 influenza A(H1N1) virus in mice.
Mice were inoculated onday 0 and treated on day 1 with the indicated antibody and dose. In each group, six mice were monitored every other day for survival.
Krause, J. C., Tsibane, T., Tumpey, T. M., Huffman, C. J., Basler, C. F., & Crowe, J. E. (2011). A broadly neutralizing human monoclonal antibody that recognizes a conserved, novel epitope on the globular head of the influenza H1N1 virus hemagglutinin. Journal of virology, 85(20), 10905-10908.
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SDS-PAGE
Figure 1 Recombinant Human Anti-IAV HA Antibody (PABL-207) in SDS-PAGE
SDS-PAGE analysis of PABL-207 in non-reduced and reduced conditions.
SEC-HPLC
Figure 2 Recombinant Human Anti-IAV HA Antibody (5J8) (PABL-207) in SEC-HPLC
The purity of PABL-207 was greater than 95% as determined by SEC-HPLC
ELISA
Figure 3 Recombinant Human Anti-IAV HA Antibody (5J8) (PABL-207) in ELISA
ELISA analysis of PABL-207 was performed by coating with Influenza A H1N1 (A/California/04/2009) Hemagglutinin / HA Protein (His Tag).
The secondary antibody: HRP-Anti-Human IgG (H+L)
WB
Figure 4 Recombinant Human Anti-IAV HA Antibody (5J8) (PABL-207) in WB
Western blot analysis of PABL-207 was performed with Influenza A H1N1 (A/California/04/2009) Hemagglutinin / HA Protein (His Tag).
Lane 1: Reducing antigen (0.1 μg)
Lane 2: Reducing antigen (0.3 μg)
Lane 3: Reducing antigen (0.6 μg)
The secondary antibody: HRP-Anti-Human IgG (H+L)
DB
Figure 5 Recombinant Human Anti-IAV HA Antibody (5J8) (PABL-207) in Dot Blot
Dot Blot analysis of PABL-207 was performed with Influenza A H1N1 (A/California/04/2009) Hemagglutinin / HA Protein (His Tag).
The secondary antibody: HRP-Anti-Human IgG (H+L)
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Specifications
- Immunogen
- Influenza a virus Hemagglutinin
- Host Species
- Human
- Derivation
- Human
- Type
- IgG
- Specificity
- Tested positive against native IAV HA
- Species Reactivity
- IAV
- Clone
- 5J8
- Applications
- FuncS
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- This antibody has been reported for use in Function Assay.
Target
- Alternative Names
- HA; hemagglutinin; haemagglutinin
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(Creative Biolabs Cat# PABL-207, RRID: AB_3111637)
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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|---|---|---|---|
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| PABL-204 | Recombinant Human Anti-IAV HA Antibody (39.29) | Neut, FuncS | IgG |
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| PSBL-207 | Recombinant Human Anti-IAV HA Antibody scFv Fragment (5J8) | WB, ELISA, FuncS | scFv |
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| PFBL-203 | Recombinant Human Anti-IAV HA Antibody Fab Fragment (3.1) | WB, ELISA, FuncS | Fab |
| PFBL-204 | Recombinant Human Anti-IAV HA Antibody Fab Fragment (39.29) | Neut, FuncS | Fab |
| PFBL-205 | Recombinant Human Anti-IAV HA Antibody Fab Fragment (1957) | WB, Neut, FuncS | Fab |
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| PFBL-207 | Recombinant Human Anti-IAV HA Antibody Fab Fragment (5J8) | WB, ELISA, FuncS | Fab |
| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| MHC-YF096 | DRB1*01:01/Influenza A HA (PKYVKQNTLKLAT) MHC Monomer | MHC Multimer | |
| MHC-YF098 | DRB1*04:01/Influenza A HA (PKYVKQNTLKLAT) MHC Monomer | MHC Multimer | |
| MHC-YF101 | DRB1*04:02/Influenza A HA (PKYVKQNTLKLAT) MHC Monomer | MHC Multimer | |
| MHC-YF1498 | BV421-DRB1*04:02/Human HA (PKYVKQNTLKLAT) MHC Tetramer | FCM | |
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